The Potential Effect of Ginger as an Adjuvant Treatment in Mice with Induced Multiple Sclerosis: Histological and Physiological Studies

Authors

Abdulkader M. A. Shaikh Omar, Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia AND Princess Najlaa Bint Saud Al-Saud Center for Excellence Research in Biotechnology, King Abdulaziz University, Jeddah, Saudi ArabiaFollow
Salim M. El-hamidy, Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia AND Princess Najlaa Bint Saud Al-Saud Center for Excellence Research in Biotechnology, King Abdulaziz University, Jeddah, Saudi Arabia
Dalia Mohammed Kenani, Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
Safa Hassan Qahl, Department of Biology, Faculty of Science, Jeddah University, Jeddah, Saudi Arabia
Ghadah Alshahrani, Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

Abstract

Objectives: Multiple sclerosis (MS) is an autoimmune disorder of the central nervous system that affect liver and kidneys. Newer treatments for MS were developed such as drug Ibudilast (IBD) and natural plants such as 6-Shogaols (Ginger components). The current study investigates the protective effects of IBD, 6-Shogaols, and their combination on the liver and kidneys of MS mice model.

Methods: Thirty-five mice were used. The study duration was 9 weeks; 5 weeks for demyelination induction by Cuprizone (CPZ) followed by 4 weeks for remyelination. At demyelination, mice were assigned into; Control group (n = 10) and CPZ group (n = 25). The control group was fed a standard diet, while the CPZ group was fed 0.3% CPZ- mixed chow. At 5^th weekend, 5 mice from control and CPZ euthanized. At remyelination, CPZ group is subdivided into: CPZ, IBD (10mg/kg/day), 6-Shogaol (25mg/kg/day), 6-Shogaol and IBD. Sera used for determined liver and kidney functions. Liver and kidneys examined under light microscopy. Same procedure follows at 9^th weekend.

Results: At 5^th week, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin, urea, creatinine, cholesterol, triglyceride, very low-density lipoprotein cholesterol (VLDL-C), LDL-C increased, while albumin, proteins and high-density lipoprotein cholesterol (HDL-C) decreased in CPZ versus control. At 9^th week, ALT, AST, ALP, bilirubin, urea, creatinine, cholesterol, triglyceride, LDL-C, VLDL-C decreased, while albumin, proteins, HDL-C increased in IBD, 6-Shogaols and their combination groups versus CPZ but were significantly changed versus control. CPZ induces histological changes in liver and kidney that improved partially after treatment.

Conclusions: Cuprizone-induced liver and kidney injuries in MS mice. IBD, 6-Shogaols and their combination had partial protective effects against damage induced by CPZ.

First Page

71

Last Page

86

Recommended Citation

Omar, Abdulkader M. A. Shaikh; El-hamidy, Salim M.; Kenani, Dalia Mohammed; Qahl, Safa Hassan; and Alshahrani, Ghadah (2025) "The Potential Effect of Ginger as an Adjuvant Treatment in Mice with Induced Multiple Sclerosis: Histological and Physiological Studies," The Journal of King Abdulaziz University: Science: Vol. 35: Iss. 2, Article 5.
DOI: https://doi.org/10.64064/1658-4252.1010

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