Molecular Analysis and Biochemical Profiling of Bacteria from Domestics Dogs Saliva: A Pilot Study

Authors

Hadba Al-Amrah, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.o. Box 80203, Jeddah, 21589, Saudi ArabiaFollow
Asseel Alotiby, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.o. Box 80203, Jeddah, 21589, Saudi Arabia
Badriyah AlJuhani, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.o. Box 80203, Jeddah, 21589, Saudi Arabia
Hadeel Huri, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.o. Box 80203, Jeddah, 21589, Saudi Arabia
Noha Al garni, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.o. Box 80203, Jeddah, 21589, Saudi Arabia
Reem Aljedani, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.o. Box 80203, Jeddah, 21589, Saudi Arabia
Zeina W. Sharawi, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.o. Box 80203, Jeddah, 21589, Saudi Arabia

Abstract

Microbiota is the collection of microorganisms living in a particular environment, such as the dog’s saliva. The aim of the present work was the detection of bacteriome that plays a crucial role in various ecological processes and has a significant impact on the health and functioning of their host organisms. We collected saliva samples from five dogs. After that, identify the unknown bacterial species by inoculating samples into the Swapped Mannitol Salt Agar (MSA) and MacConkey (MAC). In addition, These bacterial isolates were identified by using morphological characteristics, biochemical tests, and partial sequencing of their 16S rRNA gene.Results] appeared in physical tests on MSA D1, D3, and D4 as staphylococcus; however, in D2, micrococcus appeared. As for MAC, it appeared in D2, D3, D4, and D5 bacillus. The bacterial isolated were identified as Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Staphylococcus pseudintermedius, and Scandinavium goeteborgense. As for antibiotic tests, samples D2-MAC, D3MAC, and D4-MAC have a higher resistance to 83% of antibiotics, such as Penicillin G, Erythromycin, and Ampicillin, while they were susceptible to Cotrimoxazole. On the other hand, D5-MAC was resistant to antibiotic. In Conclusion: This study provides valuable insights into the potential pathogenicity of bacteria isolated from dog saliva. In addition, their antibiotic resistance profiles highlight the need for prudent antibiotic use in human and veterinary medicine.

First Page

46

Last Page

56

Recommended Citation

Al-Amrah, Hadba; Alotiby, Asseel; AlJuhani, Badriyah; Huri, Hadeel; garni, Noha Al; Aljedani, Reem; and Sharawi, Zeina W. (2025) "Molecular Analysis and Biochemical Profiling of Bacteria from Domestics Dogs Saliva: A Pilot Study," The Journal of King Abdulaziz University: Science: Vol. 35: Iss. 1, Article 5.
DOI: https://doi.org/10.64064/1658-4252.1004

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